• We derived ESC lines from

  2018-10-22

  

  We derived ESC lines from blastocysts of the double transgenic mouse line Mlc2v-Cre; Rosa26-Yfp, in which Cre and Yfp are specifically knocked into the Mlc2v and Rosa26 gene loci, respectively. Mlc2v-Cre-mediated removal of a stop sequence in the Rosa26-YFP reporter line results in expression of YFP

• Mammalian mitochondria are composed of approximately differe

  2018-10-22

  

  Mammalian mitochondria are composed of approximately 1000 different proteins, but only 13 proteins are encoded by the mitochondrial genome. We selected Atp5g, Atp8 and Ucp2 as protein markers of the mitochondrial genome. We observed progressively higher expression of Ucp2, Atp5g and Atp8 in hiPSCs,

• Similar to our observation that Nanog

  2018-10-22

  

  Similar to our observation that Nanog−/− iPSCs could give rise to chimeric animals, Nanog null ESCs have been shown to contribute to the three germ layers (Chambers et al., 2007). However, in the case of ESC chimeras, Nanog−/− GFP+ K03861 were not detected in the germline after E12.5. Because the C

• br Introduction Epidermal stem cells SCs are

  2018-10-22

  

  Introduction Epidermal stem Phenyl sulfate (SCs) are localized in several well-defined cellular compartments with the best-studied being an anatomical area of the hair follicle (HF) known as “bulge” (Blanpain and Fuchs, 2009; Cotsarelis et al., 1990; Jaks et al., 2010). This unique anatomical sit

• We found seven published clinical trials

  2018-10-22

  

  We found seven published clinical trials studying the effects of MSC transplantation in patients with liver cirrhosis (listed in Table 2). A pilot phase 1 study published in 2007 included four patients with decompensated liver cirrhosis (Mohamadnejad et al., 2007). Cells were derived from iliac cres

• Because SAPK mediates hyperosmolar stress induced

  2018-10-20

  

  Because SAPK mediates hyperosmolar stress-induced Hand1 and Hand1-dependent PL1 (Awonuga et al., 2011), we tested whether stress-induced lineage choice during hypoxia was SAPK-dependent. Since hyperosmolar stress induces the first lineage but not later lineages from TSC (Liu et al., 2009), and SAPK

• SCR 7 manufacturer The myocardium lacks the basal apical ori

  2018-10-20

  

  The myocardium lacks the basal–apical orientation typical of epithelial organs making it difficult to delineate the precise localization of CSC niches. The epicardial lining has been employed to define anatomically several classes of niches in the adult heart (Castaldo et al., 2008; Di Meglio et al.

• Recapitulating developmental signaling pathways in different

  2018-10-20

  

  Recapitulating developmental signaling pathways in differentiated cardiomyocytes has the potential to stimulate proliferation. Under this premise, developmental signaling pathways should be present in differentiated cardiomyocytes and available for activation. For example, developmentally expressed

• br Materials and methods br Results br Discussion The use

  2018-10-20

  

  Materials and methods Results Discussion The use of hNPCs for disease modeling, drug screening, or cell therapies requires the development of efficacious and cost-effective defined substrates for their expansion and neuronal differentiation. In this study, we employed an unbiased, high-thro

• One most recent study first reported the feasibility of usin

  2018-10-20

  

  One most recent study first reported the feasibility of using undifferentiated hPSC 69 9 for cardiac differentiation in suspension by small molecules modulating Wnt pathway (Kempf et al., 2014). With a fixed differentiation condition without controlling cell aggregate size, the authors show cardiac

• pppa After comparing the lymph angiogenic effects

  2018-10-20

  

  After comparing the (lymph)angiogenic effects of HLE and HLF, UVB-induced changes in their protein expression patterns were also assessed. As shown by the protein array assessment, following UVB irradiation of HLF pppa amphiregulin, uPA (a strong promoter of angiogenesis (Oh et al., 2003) the expres

• Immunohistochemical studies revealed increase in the proport

  2018-10-20

  

  Immunohistochemical studies revealed increase in the proportion of CD44highCD24low cell type from low in normal tissues to quite high in tumours. This is interesting as we know that Cancer Stem Cells are the actual tumour initiating subpopulation and can expand depending on its evolutionary landscap

• Phos-tag br Conclusions The following are the

  2018-10-20

  

  Conclusions The following are the supplementary data relate to this article. Disclosure of potential conflicts of interest Acknowledgments We thank Fondazione Roma for continuous support. We also acknowledge Prof. Mara Riminucci, Prof. Luca Pierelli, Dr. C. Murdoch, Dr. Paola Iudicone and

• br Methods br Results br Discussion The structural and funct

  2018-10-20

  

  Methods Results Discussion The structural and functional immaturity of the differentiated cardiomyocytes are major obstacles for developing hPSC-VCM-based cell replacement therapies (Kong et al., 2010; van den Heuvel et al., 2014). Although a number of studies showed that the overall matura

• Identification of the key populations that give rise to each

  2018-10-20

  

  Identification of the key populations that give rise to each wave of embryonic hematopoiesis may provide critical insights into the relationship between primitive and definitive hematopoiesis. However, the surface markers used to isolate adult HSCs and downstream stages have proven unreliable for id

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